多溴蛋白1(PBRM1)重组蛋白

YB437Hu031
Polybromo Polybromo Polybromo Polybromo 1 (PBRM1) (PBRM1) (PBRM1) (PBRM1)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
5th Edition (Revised in January, 2013)
 多溴蛋白1(PBRM1)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Polybromo 1
Synonyms: Synonyms: Synonyms: Synonyms: PBRM1, BAF180, PB1
Species: Species: Species: Species: Human
Size: 100µg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Nucleus.
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
 多溴蛋白1(PBRM1)重组蛋白Residues: Residues: Residues: Residues: Met1~Ala306 (Accession # Q86U86), with
N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 63kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by
the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 62.1kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.5
Human PBRM1
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
 多溴蛋白1(PBRM1)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
GST S GS G H H H HH HSA G LVP R GST AIG MKET AAAK FE R Q HM DSP D LG T L EV
LFQGP LGSEF - MGSKRRRATS PSSSVSGDF D DGHHSVSTPG PSRKRRR LSN
LPTVDPIAVC HELYNTIRDY KDEQGRLLCE LFIRAPKRRN QPDYYEVVSQ PIDLMKIQQK
LKMEEYDDVN LLTADFQLLF NNAKSYYKPD SPEYKAACKL WDLYLRTRNE FVQKGEADDE
DDDEDGQDNQ GTVTEGSSPA YLKEILEQLL EAIVVATNPS GRLISELFQK LPSKVQYPDY
YAIIKEPIDL KTIAQRIQNG SYKSIHAMAK DIDLLAKNAK TYNEPGSQVF KDANSIKKIF
YMKKAEIEHH EMAKSSLRMR TPSNLA