Recombinant Human Interferon-gamma
产品名称： Recombinant Human Interferon-gamma
英文名称： Immune Interferon, type II interferon, T cell interferon, MAF
产品编号： IC-106-06
产品价格： null
产品产地： 美国
品牌商标： XY-Bioscience
更新时间： 2023-08-17T09:55:27
使用范围： > 98 % by SDS-PAGE and HPLC analyses.
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Recombinant Human Interferon-gamma
| Synonyms | Immune Interferon, type II interferon, T cell interferon, MAF |
| Accession | P01579 |
| GeneID | 3458 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids. |
| Quantity | 5µg/100µg/1mg |
| AA Sequence | MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15-0.80 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 5.0, with 3 % Trehalose. |
| Endotoxin | Less than 0.01 EU/µg of rHuIFN-γ GMP as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage | This GMP product can be for research use or further manufacturing use. |
| Quality statement | The manufacture and testing of this product is in compliance with ICH Q7a guidelines. |
| Reference | 1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, 2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55. 3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56. 4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21. |
| Background | Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments TH1 cell expansion and may be required for TH1 cell differentiation. |