LIM和SH3蛋白1(LASP1)重组蛋白

YB738Hu013
Recombinant LIM And SH3 Protein 1 (LASP1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 LIM和SH3蛋白1(LASP1)重组蛋白[ PROPERTIES ]
Residues: Met1~Ile261
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q14847
Host: E. coli
Subcellular Location: Cytoplasm, cytoskeleton.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 LIM和SH3蛋白1(LASP1)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.6
Predicted Molecular Mass: 33.4kDa
Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
 LIM和SH3蛋白1(LASP1)重组蛋白Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MNP N CA RC GK IVY P TE K V N C LDKFW HK A CF HCE T CKM T L N MK NY K GYE K K
PYCNAHYPKQ SFTMVADTPE NLRLKQQSEL QSQVRYKEEF EKNKGKGFSV VADTPELQRI
KKTQDQISNI KYHEEFEKSR MGPSGGEGME PERRDSQDGS SYRRPLEQQQ PHHIPTSAPV
YQQPQQQPVA QSYGGYKEPAAPVSIQRSAP GGGGKRYRAV YDYSAADEDE VSFQDGDTIV
NVQQIDDGWM YGTVERTGDT GMLPANYVEA I