CDKN1B (phospho T157) polyclonal antibody
产品名称： CDKN1B (phospho T157) polyclonal antibody
英文名称： CDKN1B (phospho T157) polyclonal antibody
产品编号： PAB0559
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDKN1B.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T157 of human CDKN1B.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Methylation status analysis of cell cycle regulatory genes (p16INK4A, p15INK4B, p21Waf1/Cip1, p27Kip1 and p73) in natural killer cell disorders.
Kawamata N, Inagaki N, Mizumura S, Sugimoto KJ, Sakajiri S, Ohyanagi-Hara M, Oshimi K.Eur J Haematol. 2005 May;74(5):424-9.
- 2.
- BCR-ABL induces the expression of Skp2 through the PI3K pathway to promote p27Kip1 degradation and proliferation of chronic myelogenous leukemia cells.
Andreu EJ, Lledo E, Poch E, Ivorra C, Albero MP, Martinez-Climent JA, Montiel-Duarte C, Rifon J, Perez-Calvo J, Arbona C, Prosper F, Perez-Roger I.Cancer Res. 2005 Apr 15;65(8):3264-72.
- 3.
- The tumor-specific hyperactive forms of cyclin E are resistant to inhibition by p21 and p27.
Wingate H, Zhang N, McGarhen MJ, Bedrosian I, Harper JW, Keyomarsi K.J Biol Chem. 2005 Apr 15;280(15):15148-57. Epub 2005 Feb 10.
- Applications
- Western Blot (Cell lysate)
- The CDKN1B (phospho T157) polyclonal antibody (Cat # PAB0559) is used in Western blot to detect Phospho-CDKN1B-T157 in HL-60 tissue lysate
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDKN1B (phospho T157) polyclonal antibody (Cat # PAB0559) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 1027
- Protein Accession#:
- NP_004055;P46527
- Gene Name:
- CDKN1B
- Gene Alias:
- CDKN4,KIP1,MEN1B,MEN4,P27KIP1
- Gene Description:
- cyclin-dependent kinase inhibitor 1B (p27, Kip1)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq
- Other Designations:
- cyclin-dependent kinase inhibitor 1B
- Related Disease
- Acromegaly
- Adenocarcinoma
- Adenocarcinoma, Follicular
- Alzheimer Disease
- Alzheimer disease
- Ataxia telangiectasia
- Atherosclerosis
- Breast cancer
- Breast Neoplasms
- Carcinoma
- Carcinoma, Ductal, Breast
- Carcinoma, Hepatocellular
- Carcinoma, Papillary, Follicular
- Carcinoma, Squamous Cell
- Cardiovascular Diseases
- Chromosome Aberrations
- Cognition
- Colon cancer
- Colorectal Neoplasms