MOS (Human) Recombinant Protein
产品名称： MOS (Human) Recombinant Protein
英文名称： MOS (Human) Recombinant Protein
产品编号： P5602
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 所在区域 : 台湾
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- Specification
- Product Description:
- Human MOS (NP_005363.1, 1 a.a. - 346 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 65
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 77 % by SDS-PAGE/CBB staining
- Activity:
- The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG). Substrate: MAP2K1 [inactive mutant]. ATP: 100 uM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 4342
- Protein Accession#:
- NP_005363.1
- Gene Name:
- MOS
- Gene Alias:
- MGC119962,MGC119963,MSV
- Gene Description:
- v-mos Moloney murine sarcoma viral oncogene homolog
- Omim ID:
- 190060
- Gene Ontology:
- Hyperlink
- Gene Summary:
- MOS is a serine/threonine kinase that activates the MAP kinase cascade through direct phosphorylation of the MAP kinase activator MEK (MAP2K1; MIM 176872) (Prasad et al., 2008 [PubMed 18246541]).[supplied by OMIM
- Other Designations:
- Oncogene MOS, Moloney murine sarcoma virus