MAP2K3 (Human) Recombinant Protein
产品名称： MAP2K3 (Human) Recombinant Protein
英文名称： MAP2K3 (Human) Recombinant Protein
产品编号： P5584
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Human MAP2K3 (NP_659731.1, 1 a.a. - 347 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 66
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 84 % by SDS-PAGE/CBB staining
- Activity:
- The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). Substrate: p38a (9-352) [inactive mutant]. ATP: 100 uM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 5606
- Protein Accession#:
- NP_659731.1
- Gene Name:
- MAP2K3
- Gene Alias:
- MAPKK3,MEK3,MKK3,PRKMK3
- Gene Description:
- mitogen-activated protein kinase kinase 3
- Omim ID:
- 602315
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq
- Other Designations:
- MAP kinase kinase 3,MAPK/ERK kinase 3,OTTHUMP00000166044,dual specificity mitogen activated protein kinase kinase 3