CAPNS1 monoclonal antibody (M01), clone 3C4
产品名称： CAPNS1 monoclonal antibody (M01), clone 3C4
英文名称： CAPNS1 monoclonal antibody (M01), clone 3C4
产品编号： H00000826-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant CAPNS1.
- Immunogen:
- CAPNS1 (AAH00592, 172 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- KRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLYNMIIRRYSDESGNMDFDNFISCLVRLDAMFRAFKSLDKDGTGQIQVNIQE
- Host:
- Mouse
- Reactivity:
- Human, Rat
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.42 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Overexpression of a Minimal Domain of Calpastatin Suppresses IL-6 Production and Th17 Development via Reduced NF-kB and Increased STAT5 Signals.
Iguchi-Hashimoto M, Usui T, Yoshifuji H, Shimizu M, Kobayashi S, Ito Y, Murakami K, Shiomi A, Yukawa N, Kawabata D, Nojima T, Ohmura K, Fujii T, Mimori T.PLoS One. 2011;6(10):e27020. Epub 2011 Oct 27.
- 2.
- The calpain inhibitor calpeptin prevents bleomycin-induced pulmonary fibrosis in mice.
Tabata C, Tabata R, Nakano T.Clin Exp Immunol. 2010 Sep 15. doi: 10.1111/j.1365-2249.2010.04257.x. [Epub ahead of print]
- 3.
- Vitamin E and C supplementation does not ameliorate muscle dysfunction following anterior cruciate ligament surgery.
Barker T, Leonard SW, Hansen J, Trawick RH, Ingram R, Burdett G, Lebold KM, Walker JA, Traber MG.Free Radic Biol Med. 2009 Sep 11. [Epub ahead of print]
- Applications
- Western Blot (Cell lysate)
- CAPNS1 monoclonal antibody (M01), clone 3C4. Western Blot analysis of CAPNS1 expression in PC-12 ( Cat # L012V1 ).
- Protocol Download
- Western Blot (Cell lysate)
- CAPNS1 monoclonal antibody (M01), clone 3C4 Western Blot analysis of CAPNS1 expression in A-431 ( Cat # L015V1 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of CAPNS1 expression in transfected 293T cell line by CAPNS1 monoclonal antibody (M01), clone 3C4.
Lane 1: CAPNS1 transfected lysate(28.3 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to CAPNS1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to CAPNS1 on HeLa cell. [antibody concentration 20 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged CAPNS1 is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of CAPNS1 over-expressed 293 cell line, cotransfected with CAPNS1 Validated Chimera RNAi ( Cat # H00000826-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAPNS1 monoclonal antibody (M01), clone 3C4 (Cat # H00000826-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunofluorescence
- enlarge
- Sandwich ELISA (Recombinant protein)
- enlarge
- ELISA
- RNAi Knockdown (Antibody validated)
- enlarge
- Entrez GeneID:
- 826
- GeneBank Accession#:
- BC000592
- Protein Accession#:
- AAH00592
- Gene Name:
- CAPNS1
- Gene Alias:
- 30K,CALPAIN4,CANP,CANPS,CAPN4,CDPS
- Gene Description:
- calpain, small subunit 1
- Omim ID:
- 114170
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. This gene encodes a small subunit common to both calpain I and II and is associated with myotonic dystrophy. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
- Other Designations:
- calcium-activated neutral proteinase,calcium-dependent protease, small subunit,calpain 4, small subunit (30K),calpain, small polypeptide
- Related Disease