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上海信裕生物科技有限公司
KRAS-2A-specific 抗体

KRAS-2A-specific 抗体

商家询价

产品名称: KRAS-2A-specific 抗体

英文名称: KRAS-2A-specific Antibody

产品编号: XY16156-1

产品价格: null

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: WB,ELISA,IHC-P,IHC-F,IF

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 KRAS-2A-SPECIFIC 抗体

Applications
In-house applications ELISA, WB, IHC, FC, IF     Application not tested? Please see our Guarantee.
Species specificity Human; other species not tested.
Images
Prev12NextView more validation data
  • 16156-1-AP;SH-SY5Y cellSH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16156-1-AP(KRAS-2A-specific antibody) at dilution of 1:500
  • 16156-1-AP;Lung cancerImmunohistochemical of paraffin-embedded human lung cancer using 16156-1-AP(KRAS-2A-specific antibody) at dilution of 1:50 (under 10x lens)
  • 16156-1-AP;Lung cancerImmunohistochemical of paraffin-embedded human lung cancer using 16156-1-AP(KRAS-2A-specific antibody) at dilution of 1:50 (under 40x lens)
  • 16156-1-AP;SH-SY5Y cellsImmunofluorescent analysis of ( -20℃ Acetone ) fixed SH-SY5Y cells using 16156-1-AP( KRAS-2A-specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 16156-1-AP;HeLa cells1X10^6 HeLa cells were stained with 0.2ug KRAS-2A-specific antibody (16156-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in SH-SY5Y cells
Positive IHC detected in Human lung cancer tissue, human colon cancer tissue
Positive IF detected in SH-SY5Y cells
Positive FC detected in HeLa cells
Recommended dilution WB : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500
 KRAS-2A-SPECIFIC 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information KRAS-2A-SPECIFIC 抗体
Immunogen Peptide Full name V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular
weight
21.7 kDa Observed molecular 
weight
21-24 kDa
GenBank accession 
number
NM_033360 Gene ID (NCBI) 3845
Gene symbol KRAS Synonyms C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS2B speicifc, KRAS4A, Krasa, Krasb, NS3, RASK2
Background

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2A, which also named as KRAS-4A . KRAS-2A-SPECIFIC 抗体

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