This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Growth Hormone Releasing Hormone (GHRH) has  been  pre-coated  onto  a  microplate.  A  competitive  inhibition  reaction  is  launched  between Horseradish Peroxidase (HRP) labeled Growth Hormone Releasing Hormone (GHRH) and unlabeled Growth Hormone Releasing Hormone (GHRH) (Standards or samples) with the pre-coated antibody specific for Growth Hormone Releasing Hormone (GHRH). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of  Growth Hormone Releasing Hormone (GHRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of  Growth Hormone Releasing Hormone (GHRH) in the sample.