Biotinylated Recombinant Human PD-1/PDCD1 Protein, His-Avi Tag
产品名称： Biotinylated Recombinant Human PD-1/PDCD1 Protein, His-Avi Tag
英文名称： Biotinylated Recombinant Human PD-1/PDCD1 Protein, His-Avi Tag
产品编号： 93579ES25
产品价格： 0
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2024-12-10T11:28:44
使用范围： null
- 联系人 : 李自转
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- 邮编 : 200030
- 所在区域 : 上海
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产品描述
PD-1 a receptor expressed on T cells responsible for modulating T cell activation. PD-1 is classified as an immune checkpoint inhibitory receptor. PD1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes. Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion.
产品性质
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别名 |
PDCD1; PD1; CD279; SLEB2; PD-1; Programmed Death-1 receptor |
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Uniprot No. |
Q15116.1 |
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表达区间及表达系统 |
Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Leu25-Gln167. |
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分子量 |
Approximately 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
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纯度 |
> 95% as determined by SDS-PAGE and HPLC. |
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活性 |
ELISA Data: Immobilized Biotinylated Human PD-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. SPR Data: Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). |
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内毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂 |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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复溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 TUNEology 波长可调检测卡盒-->
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