ATAD5 polyclonal antibody
产品名称： ATAD5 polyclonal antibody
英文名称： ATAD5 polyclonal antibody
产品编号： PAB10222
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of ATAD5.
- Immunogen:
- A synthetic peptide corresponding to amino acids 63-76 of human ATAD5.
- Host:
- Rabbit
- Reactivity:
- Chimpanzee, Dog, Human
- Specificity:
- The following proteins should be considered to be highly homologous to Elg1 when using this antibody: human chromosome fragility associated gene 1 (NP_079133), hypothetical dog protein FLJ12735 (XP_548276), hypothetical chimpanzee protein FLJ12735 (XP_511388), and a hypothetical human protein of 1224 residues (CAH10412). All show 100% homology to the immunogen.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:3000-1:15000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability.
Kanellis P, Agyei R, Durocher D.Curr Biol. 2003 Sep 16;13(18):1583-95.
- 2.
- A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions.
Jenne DE, Tinschert S, Stegmann E, Reimann H, Nurnberg P, Horn D, Naumann I, Buske A, Thiel G.Genomics. 2000 May 15;66(1):93-7.
- Applications
- Western Blot (Cell lysate)
- Western blot using ATAD5 polyclonal antibody (Cat # PAB10222) shows detection of a band ~120 KDa corresponding to human ATAD5 (arrowhead) in various cell lysates.
Lanes contain ~ 5 ug of HeLa nuclear extract (1), HeLa (2), A-431 (3), Jurkat (4) and HEK293 (5) whole cell lysates.
After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500.
The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2m exposure time.
The expected molecular weight of ATAD5 is 120 KDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 KDa.
The 50 KDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins.
Both the 120 KDa and 50 KDa bands are not observed when antibody is pre-incubated with peptide (datanot shown).