纽蛋白(VCL)重组蛋白

YB839Mu024
Recombinant Vinculin (VCL)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
纽蛋白(VCL)重组蛋白[ PROPERTIES ]
Residues: Lys1020~Gln1134 (Accession # P18206),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cytoplasm. cytoskeleton.
Cell junction. adherens junction. Cell membrane;
Peripheral membrane protein; Cytoplasmic side.
focal adhesion. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
纽蛋白(VCL)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM
Tris, 500mM NaCl, pH8.0, containing 1mM EDTA,
5% trehalose, and preservative. Predicted isoelectric point: 9.0
Predicted Molecular Mass: 14.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
纽蛋白(VCL)重组蛋白which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF -K DIAKASDEVT RLAKEVAKQC TDKRIRTNLL QVCERIPTIS
TQLKILSTVK ATMLGRTNIS DEESEQATEM LVHNAQNLMQ SVKETVREAE AASIKIRTDA
GFTLRWVRKT PWYQ