PPPDE1/PNAS4抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
PPPDE1/PNAS4抗体

PPPDE1/PNAS4抗体

商家询价

产品名称: PPPDE1/PNAS4抗体

英文名称: PPPDE1/PNAS4Antibody

产品编号: XY 20517-1

产品价格: null

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: WB,ELISA,IHC-P,IHC-F,IF

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 PPPDE1/PNAS4抗体Applications

In-house applications ELISA, WB, IHC, IF, IP     Application not tested? Please see our Guarantee.
Published applications IF, IHC, WB Publications 3 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human, mouse
Images
Prev12NextView more validation data
  • 20517-1-AP;A549 cellsWB result of PPPDE1 antibody (20517-1-AP, 1:1000) with A549 cells transfected with si-control and si-PPPDE1.
  • 20517-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 20517-1-AP(PPPDE1 antibody) at dilution of 1:500
  • 20517-1-AP;human lung cancerImmunohistochemical of paraffin-embedded human lung cancer using 20517-1-AP(PPPDE1 antibody) at dilution of 1:50 (under 10x lens)
  • 20517-1-AP;human lung cancerImmunohistochemical of paraffin-embedded human lung cancer using 20517-1-AP(PPPDE1 antibody) at dilution of 1:50 (under 40x lens)
  • 20517-1-AP;HepG2Immunofluorescent analysis of HepG2 cells, using PPPDE1 antibody 20517-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).
  • 20517-1-AP;A549 cellsIP Result of anti-PPPDE1 (IP:20517-1-AP, 4ug; Detection:20517-1-AP 1:500) with A549 cells lysate 2800ug.
Positive WB detected in HeLa cells, A549 cells
Positive IP detected in A549 cells
Positive IHC detected in Human lung cancer tissue
Positive IF detected in HepG2 cells
Recommended dilution WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
 PPPDE1/PNAS4抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information PPPDE1/PNAS4抗体
Immunogen PPPDE1/PNAS4 fusion protein ag14352 Full name PPPDE peptidase domain containing 1
Calculated molecular
weight
194aa,21 kDa Observed molecular 
weight
23 kDa
GenBank accession 
number
BC004485 Gene ID (NCBI) 51029
Gene symbol PPPDE1 Synonyms DESI2, Desumoylating isopeptidase 2, FAM152A, PNAS 4, PPPDE1
Background

PPPDE peptidase domain 1 (PPPDE1), also named as PNAS4, is a highly conserved gene ubiquitously expressed in various tissues. PPPDE1 has been identified as a novel pro-apoptotic gene activated during the early response to DNA damage which is thought to play a critical role in cellular function regarding the maintenance of genomic integrity. This antibody specifically recognized endogenous PPPDE1, which has been confirmed by siRNA test. PPPDE1/PNAS4抗体

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