多配体蛋白聚糖4(SDC4)检测试剂盒(酶联免疫吸附试验法)
产品名称: 多配体蛋白聚糖4(SDC4)检测试剂盒(酶联免疫吸附试验法)
英文名称: ELISA Kit for Syndecan 4 (SDC4)
产品编号: CKRG3530
产品价格: 0
产品产地: 中美合资
品牌商标: 天津科瑞杰
更新时间: null
使用范围: null
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syndecan 4 (SDC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Syndecan 4 (SDC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syndecan 4 (SDC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syndecan 4 (SDC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.