SPRED1 polyclonal antibody
产品名称： SPRED1 polyclonal antibody
英文名称： SPRED1 polyclonal antibody
产品编号： PAB3100
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of SPRED1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SPRED1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Germline loss-of-function mutations in SPRED1 cause a neurofibromatosis 1-like phenotype.
Brems H, Chmara M, Sahbatou M, Denayer E, Taniguchi K, Kato R, Somers R, Messiaen L, De Schepper S, Fryns JP, Cools J, Marynen P, Thomas G, Yoshimura A, Legius E.Nat Genet. 2007 Sep;39(9):1120-6. Epub 2007 Aug 19.
- 2.
- Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns.
Szafranski K, Schindler S, Taudien S, Hiller M, Huse K, Jahn N, Schreiber S, Backofen R, Platzer M.Genome Biol. 2007;8(8):R154.
- 3.
- Spreds, inhibitors of the Ras/ERK signal transduction, are dysregulated in human hepatocellular carcinoma and linked to the malignant phenotype of tumors.
Yoshida T, Hisamoto T, Akiba J, Koga H, Nakamura K, Tokunaga Y, Hanada S, Kumemura H, Maeyama M, Harada M, Ogata H, Yano H, Kojiro M, Ueno T, Yoshimura A, Sata M.Oncogene. 2006 Oct 5;25(45):6056-66. Epub 2006 May 1.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis of SPRED1 polyclonal antibody (Cat # PAB3100) (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 polyclonal antibody (Cat # PAB3100) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 161742
- Protein Accession#:
- NP_689807;Q7Z699
- Gene Name:
- SPRED1
- Gene Alias:
- FLJ33903,NFLS
- Gene Description:
- sprouty-related, EVH1 domain containing 1
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq
- Other Designations:
- EVH1/Sprouty domain containing protein,sprouty-related protein 1 with EVH-1 domain,suppressor of Ras/MAPK activation
- Gene Pathway