I.U.B.: 1.1.3.9

Galactose oxidase oxidizes galactose and some galactose derivatives in both free and polymeric forms. Oxidation occurs at the C6 position. The enzyme has a molecular weight of 68 ± 3 kDa, and the optimum pH is 7.0.

Technical Note: One A₄₂₅ unit is approximately equivalent to 0.54 µmole oxidized galactose.

Unit Definition: One Unit equals a change in absorbance at 425 nm of 1.000 per minute at 25°C, pH 6.0 using a peroxidase/o-tolidine coupled assay with galactose as the substrate. Note: Product code BGC is not suitable for immunoconjugation applications due to the presence of ammonium sulfate.